DAPI (hydrochloride) [28718-90-3]
Referentie C3362-5mg
Formaat : 5mg
Merk : APExBIO Technology
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Telefoonnummer : +1 850 650 7790
DAPI (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DAPI (hydrochloride) is a fluorescent probe which serves as a DNA-specific probe for flow cytometry, chromosome staining, as well as DNA visualization and quantitation in histochemistry and biochemistry. DAPI forms a fluorescent complex by attaching to the minor grove of consecutive (3 ~ 4 base pairs) A-T rich sequences of DNA. Although DAPI is capable of binding to other sequences of double stranded DNA, or double stranded RNA, these complexes are much less fluorescent. DAPI can be used alone for cell cycle studies of drug effects, or in combination with another fluorochrome for multi-parameter cell analysis. One of the most popular stains combines DAPI with sulforhodamine (SR 101) for simultaneous measurement of DNA and protein content in cells. DAPI can be used on either fixed or live cells, but its low permeability in live cells suggests that higher concentrations should be used.
References:
1. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotechnic & Histochemistry, 1995, 70(5): 220-233.
2. Zink D, Sadoni N, Stelzer E. Visualizing chromatin and chromosomes in living cells. Methods, 2003, 29(1): 42-50.
- 1. Mei Liu, et al. "Shear Bond Strength to Enamel, Mechanical Properties and Cellular Studies of Fiber-Reinforced Composites Modified by Depositing SiO2 Nanofilms on Quartz Fibers via Atomic Layer Deposition." Int J Nanomedicine. 2024 Mar 5:19:2113-2136. PMID: 38476282
- 2. ZHOU Qiyue, HONG Gaoying, et al. "Characterization and biocompatibility analysis of different silanes coupling c (RGDfK) cyclic peptide on titanium surfaces." J Prev Treat Stomatol Dis, 2022, 30(6): 398⁃405.
- 3. Liang Y, Wang J. "Sorafenib-Induced Exosome Secretion Promotes Che-motherapy Resistance in Hepatoma Cell Line." Japanese J Gstro Hepato. 2021; V6(12): 1-7.
| Storage | Store at -20°C |
| M.Wt | 350.25 |
| Cas No. | 28718-90-3 |
| Formula | C16H17Cl2N5 |
| Solubility | insoluble in EtOH; ≥10 mg/mL in H2O; ≥53.3 mg/mL in DMSO |
| Chemical Name | 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |