Diacylglycerol ELISA Kit
Referentie OKEH02607
Formaat : 96Wells
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | ELISA-Comp | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Diacylglycerol ELISA Kit (OKEH02607) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Diacylglycerol antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Diacylglycerol and incubated. The Diacylglycerol found in the sample or standards competes with the biotinylated Diacylglycerol for limited binding sites on the immobilized anti-Diacylglycerol antibody. Excess unbound biotinylated Diacylglycerol and sample or standard Diacylglycerol is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Diacylglycerol captured in the well and inversely proportional to the amount of Diacylglycerol which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 96% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.5% (n = 20) Mean Inter-assay CV%: < 8.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: DAG Target Name: Diacylglycerol | ||||||||||||||||||||||
| :: | Pubchem: 6026790 | ||||||||||||||||||||||
| :: | Chemical formula: C37H70O5 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.262 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Diacylglycerol | ||||||||||||||||||||||
| Publications | DAGLα Inhibition as a Non-invasive and Translational Model of Episodic Headache. Front Pharmacol. 11, 615028 (2021). 33584293 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | DAG, Diacylglycerol, DAG, Diglyceride |
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| Protein Name | Diacylglycerol |
| Description of Target | A diglyceride, or a diacylglycerol (DAG), is a glyceride consisting of two fatty acid chains covalently bonded to a glycerol molecule through ester linkages. One example, shown on the right, is 1-palmitoyl-2-oleoyl-glycerol, which contains side-chains derived from palmitic acid and oleic acid. Diacylglycerols can also have many different combinations of fatty acids attached at both the C-1 and C-2 positions. |