Dipeptidyl Peptidase 7, BioAssay™ Kit (DPP7)

The Fluorogenic DPP7 Assay Kit is a complete assay system designed to measure activity of the purified DPP7 enzyme. It comes in a convenient 96-well format, with all the reagents necessary for 100 reactions. Based on the unique fluorogenic substrate, the Fluorescent DPP7 Activity Assay eliminates the dealing with radioactive materials and chromatography in traditional assays. Purified human recombinant DPP7 is included in the kit as a positive control. Using this kit, only one simple step, in which the fluorometric substrate is incubated with purified DPP7, is needed to analyze the DPP7 activity level. The resulting fluorescent product can then be measured with a microtiterplate fluorimeter.||Kit Components: |1. Fluorogenic DPP7 substrate (0.5mM): 1x100ul|2. DPP7 assay buffer: 1x10ml|3. 1 black plate: one plate|4. Recombinant human DPP7 for 96 reactions: 1x5ug|5. Fluorescent standard (50uM): 1x500ul ||APPLICATIONS: |Suitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.||Storage and Stability:|Store buffer and standard at -20°C. Store enzyme and substrate at -70°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.