Dipeptidyl Peptidase 8, BioAssay™ Kit (DPP8)

Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP8 shares homology and substrate recognition patterns with DPP4 and like DPP4, is thought to play a role in T-cell activation and immune function. The DPP8 Fluorescent Activity Assay kit is designed to measure DPP8 activity using purified DPP8 or cell extracts containing DPP8. It comes in a convenient 96-well format, with purified DPP8 enzyme, DPP8 substrate, and DPP8 assay buffer for 100 enzyme reactions. The key to the DPP8 Fluorescent Activity Assay is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The DPP8 fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.||APPLICATIONS: |Suitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.||Kit Components: |*D8065-31P1: DPP8 Enzyme Recombinant Human, 1x5ug|D8065-31P2: DPP Assay Buffer: 1x10ml|*D8065-31P3: Fluorogenic DPP Substrate 1, 0.5mM in DMSO, 1x100ul|D8065-31P4: AMC Fluorescent standard (50uM), 1x500ul|D8065-31P5: Microtiter Plate, 1x96wells||Storage and Stability:|Store *D8065-31P1 and *D8065-31P3 at -70°C. Store D8065-31P5 at RT. Store other components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.