I.U.B.: 3.4.24.28|A metallo, neutral protease exhibits mild proteolytic action that makes the enzyme especially suitable for the preparation of primary and secondary (subcultivation) cell culture. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications.||Unit Definition: |One Unit releases Folin positive amino acids equivalent to 1umole tyrosine per minute from casein at 37°C, pH 7.5.||Specific Activity: |≥4 units per mg dry weight||Protein:|As Reported||Units/mg Protein:|As Reported||Caseinase:|As Reported||Absorbance A280:|As Reported||Endotoxin:|As Reported||Storage and Stability: |Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.