DL-dithiothreitol [3483-12-3]
Referentie HY-15917-5g
Formaat : 5g
Merk : MedChemExpress
DL-dithiothreitol (DTT) is a reducing agent. DL-dithiothreitol forms a stable six-membered ring with an internal disulfide bond once oxidized.
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DL-dithiothreitol Chemical Structure
CAS No. : 3483-12-3
This product is a controlled substance and not for sale in your territory.
Based on 12 publication(s) in Google Scholar
Other Forms of DL-dithiothreitol:
- L-Dithiothreitol In-stock
- DL-dithiothreitol-d6 In-stock
- DL-dithiothreitol-d10 Obtenir un devis
- DL-dithiothreitol-d10-1 Obtenir un devis
Description |
DL-dithiothreitol (DTT) is a reducing agent. DL-dithiothreitol forms a stable six-membered ring with an internal disulfide bond once oxidized. |
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In Vitro |
DL-dithiothreitol is widely used in biochemistry works to reduce dissulfide bridges, protect biomolecules, in sample preparation, and to denature proteins before electrophoresis analysis (SDS-PAGE). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
154.25 |
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Formule |
C4H10O2S2 |
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CAS No. |
3483-12-3 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
SC[C@@H](O)[C@H](O)CS |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
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Solvant et solubilité |
In Vitro:
H2O : ≥ 200 mg/mL (1296.60 mM) DMSO : 100 mg/mL (648.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Pureté et documentation | |||||||||||||
Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 6.4830 mL | 32.4149 mL | 64.8298 mL | 162.0746 mL |
5 mM | 1.2966 mL | 6.4830 mL | 12.9660 mL | 32.4149 mL | |
10 mM | 0.6483 mL | 3.2415 mL | 6.4830 mL | 16.2075 mL | |
15 mM | 0.4322 mL | 2.1610 mL | 4.3220 mL | 10.8050 mL | |
20 mM | 0.3241 mL | 1.6207 mL | 3.2415 mL | 8.1037 mL | |
25 mM | 0.2593 mL | 1.2966 mL | 2.5932 mL | 6.4830 mL | |
30 mM | 0.2161 mL | 1.0805 mL | 2.1610 mL | 5.4025 mL | |
40 mM | 0.1621 mL | 0.8104 mL | 1.6207 mL | 4.0519 mL | |
50 mM | 0.1297 mL | 0.6483 mL | 1.2966 mL | 3.2415 mL | |
60 mM | 0.1080 mL | 0.5402 mL | 1.0805 mL | 2.7012 mL | |
80 mM | 0.0810 mL | 0.4052 mL | 0.8104 mL | 2.0259 mL | |
100 mM | 0.0648 mL | 0.3241 mL | 0.6483 mL | 1.6207 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DL-dithiothreitol Related Classifications
- Others
- Others
- Biochemical Assay Reagents
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
DL-dithiothreitol3483-12-3DTT rel-(2R,3R)-1,4-Dimercapto-2,3-butanediolBiochemical Assay ReagentsInhibitorinhibitorinhibit