DNA Marker 100bp Ladder, 10 Fragments (100-1000bp), BioGenomics™

DNA Marker 100bp DNA ladder is supplied at a total DNA concentration of 0.5mg/ml and is prepared from specially designed plasmid pME-80 DNA, containing pUC, lambda phage and yeast genome sequences.||The marker yields the following 10 discrete fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100. Convenient for analysis of PCR products up to 1 kb and can be used for DNA quantification.||The bright band at 500 bp serves as the reference.||Concentration:|0.5mg DNA/ml||Storage Buffer:|10mM Tris-HCl, pH 7.6, 1mM EDTA.||6X Loading Dye Solution (L3550-01):|Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 0.15% orange, 60% glycerol, 60mM EDTA.||Prepare DNA Marker for Agarose:|1-2ul DNA Ladder (0.5-1ug)|1ul of 6X Loading Dye Solution|3-4ul ddH2O||Prepare DNA Marker for Gels Polyacrylamide:|1-2ul DNA Ladder (0.5-1ug)|0.5ul of 6X Loading Dye Solution |0.5-1.5ul ddH2O||Protocol:|1. Mix gently|2. Load on the gel|*For gels with other lane widths, the comnponents of the mixture should be scaled either up or down. Use 0.2-0.4ul (0.1-0.2ug) of DNA Ladder oer 1mm of lane.||Recommendations:|1. Do not heat before loading. |2. Dilute DNA. sample with the 6X DNA. Loading Dye: mix 1 volume of the dye solution with 5 volumes of the DNA. sample. Load same volume of DNA sample and the DNA ladder.|3. Adjust concentration to equalize the amount of DNA. in the nearest band of the ladder.|4. For visualization with other intercalating dyes, do not add the dyes into the sample, use gel staining after electrophoresis or include dyes into agarose gel to avoid aberrant DNA migration.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.||Band Concentration:|0.5ug of DNA Ladder Mix contains:|a) 45ng (9.0%) for each of the 600-1000bp fragments.|b) 115ng (23.0%) for 500 bp band |c) 40ng (8.0%) for 100-400 bp bands.