Donkey IgG (H+L chain) Rabbit Polyclonal Antibody
Referentie R1251HRP
Formaat : 2mg
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Donkey IgG (H+L chain) Rabbit Polyclonal Antibody
SKU
R1251HRP
Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP
3 Days*
| Product Data | |
| Application | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
|---|---|
| Reactivity | Donkey |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Donkey IgG whole molecule. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG and Donkey Serum. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase |
| Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Purification | Immunoaffinity Chromatography. |
| Conjugation | HRP |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.