DOPE [4004-05-1]
Referentie HY-112005-100mg
Formaat : 100mg
Merk : MedChemExpress
DOPE (dioleoylphosphatidylethanolamine) is a neutral helper lipid for cationic liposome and combines with cationic phospholipids to improve transfection efficiency of naked siRNA.
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DOPE Chemical Structure
CAS No. : 4004-05-1
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
DOPE (dioleoylphosphatidylethanolamine) is a neutral helper lipid for cationic liposome and combines with cationic phospholipids to improve transfection efficiency of naked siRNA[1]. |
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IC50 & Target |
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In Vitro |
In this study, each siRNA is encapsulated in LNP (cationic lipid-A: PEG2000-DMPE: DOPE = 47.8: 5.18: 47.1 molar percent)[1].With the neutral lipid for the LNP is DOPE, LNP exerts knock-down (KD) effecton macrophages and DCs and Liposome-containing DOPE shows fusogenicity[1].LNP/siRNA significantly suppresses CD45 protein expression at an inhibition rate of 30% on macrophages and DCs but not on monocytes, neutrophils, plasmacytoid DCs, or B cells[1].LNP has the potential to be an efficient siRNA delivery system not only for murine but also for human macrophages and DCs[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
744.03 |
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Formule |
C41H78NO8P |
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CAS No. |
4004-05-1 |
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Appearance |
Solid |
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Color |
White to light yellow |
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SMILES |
O=P(OC[C@H](OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O)(OCCN)O |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
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Solvant et solubilité |
In Vitro:
DMSO : 50 mg/mL (67.20 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Ethanol : 10 mg/mL (13.44 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: ≥98.0% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol / DMSO | 1 mM | 1.3440 mL | 6.7202 mL | 13.4403 mL | 33.6008 mL |
5 mM | 0.2688 mL | 1.3440 mL | 2.6881 mL | 6.7202 mL | |
10 mM | 0.1344 mL | 0.6720 mL | 1.3440 mL | 3.3601 mL | |
DMSO | 15 mM | 0.0896 mL | 0.4480 mL | 0.8960 mL | 2.2401 mL |
20 mM | 0.0672 mL | 0.3360 mL | 0.6720 mL | 1.6800 mL | |
25 mM | 0.0538 mL | 0.2688 mL | 0.5376 mL | 1.3440 mL | |
30 mM | 0.0448 mL | 0.2240 mL | 0.4480 mL | 1.1200 mL | |
40 mM | 0.0336 mL | 0.1680 mL | 0.3360 mL | 0.8400 mL | |
50 mM | 0.0269 mL | 0.1344 mL | 0.2688 mL | 0.6720 mL | |
60 mM | 0.0224 mL | 0.1120 mL | 0.2240 mL | 0.5600 mL |
DOPE Related Classifications
- Réactifs de dosage biochimique
- Drug Delivery
- Inflammation/Immunology
- Metabolic Enzyme/Protease
- Liposome Endogenous Metabolite