EasyPure® Plasmid MiniPrep Kit
Referentie EM101-02
Formaat : 200rxns
Merk : TransGen Biotech
EasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.
• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
Component | EM101-01 (50 rxns) | EM101-02 (200 rxns) |
Resuspension Buffer (RB) | 15 ml | 60 ml |
Lysis Buffer (LB) | 15 ml | 60 ml |
Neutralization Buffer (NB) | 20 ml | 80 ml |
Wash Buffer (WB) | 10 ml | 2×20 ml |
Elution Buffer (EB) | 5 ml | 10 ml |
RNase A (10 mg/ml) | 150 μl | 600 μl |
Mini-Plasmid Spin Column with Collection Tubes | 50 each | 2×100 each |
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