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> Ethanol Colorimetric Assay Kit

Ethanol Colorimetric Assay Kit

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Referentie E-BC-K891-M-500

Formaat : 500Assays

Merk : Elabscience

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Telefoonnummer : +1 850 650 7790

Detection Principle Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Sample Type Serum,Plasma,Wine
Detection Method Colorimetric method
Detection Instrument Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Research Area Liver And Renal Function
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.27 μmol/mL
Detection Range 0.27-10.0 μmol/mL
Precision inter-assay CV: 0.05 | intra-assay CV: 3.5%
Assay Time 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Beer (2.8% alcohol) 60-100
White wine (12% alcohol) 250-300

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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