| Description | F(ab')2 Anti-RAT IgG F(ab')2 (RABBIT) Antibody Fluorescein Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | Fluorescein (FITC) |
| FP Value | 2.9 moles Fluorescein (FITC) per mole of IgG F(ab')2 |
| Target Species | Rat |
| Clonality | Polyclonal |
Application 
| IF, FC |
| Application Note | FLISA 1:10,000-1:50,000 IF Microscopy 1:1,000-1:5,000 FlowCytometry 1:500-1:2,500 |
| Physical State | Lyophilized |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG F(ab')2 |
| Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Rat IgG F(ab')2 fragment |
| Reconstitution Volume | 2.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Thimerosal |
| Shipping Condition | Ambient |
|---|---|
| Purity | This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |