K2042-100 assays | FAD Colorimetric/Fluorometric Assay Kit Clinisciences

Description

ATP is an important source of energy within cells and can be used to measure the level of metabolism in cells. ATP levels are also positively correlated with the number of viable cells. Therefore, ATP levels can be used to measure the number of viable cells.

Luminescent ATP Cell Viability Assay Kit II is a kit that uses firefly luciferase to detect cell viability. The reagent provided in this kit contains thermal-stable firefly luciferase and a highly purified substrate luciferin. This reagent can lyse cells and release ATP, which then produces a stable luminescence signal catalyzed by luciferase. The intensity of its luminescence signal is proportional to the number of viable cells.

This kit is a lyophilized powder packaging version of ATP luminescence cell viability assay kit I, and the detection effect of the two is basically the same. The ATP Luminescent Cell Viability Kit I provides a ready-to-use solution for greater convenience, but cannot be stored at -20°C for long-term storage. The kit is in lyophilized powder form and requires dissolving the substrate in the buffer provided prior to use, but the reagent is stable at -20°C. Compared to other cell viability reagents, such as MTT, CCK-8, Alamar Blue, and Calcein-AM, this reagent offers higher sensitivity and shorter detection times. In addition, the reagent has good linearity in 10-30,000 cells. This kit has been tested to be as effective as CellTiter-Glo^® Luminescent Cell Viability Assay (CTG).

Figure 1: Schematic diagram of the detection of this kit

Figure 2: Validation diagram of this kit. The left picture shows the effect of this kit and similar foreign products (Competitor P) for detecting cell proliferation, and the right picture shows the effect of this kit on the ATP standard curve

Quality Control

Protocol

Components and Storage

Components	K2042-100 T	K2042-1000 T
Luminescent ATP Cell Viability Assay Substrate	1 vial	2 vials
Luminescent ATP Cell Viability Assay Buffer	10 mL	2 x 50 mL
Store the kit at -20°C away from light, stable for at least 1 year. Prepared Luminescent ATP Cell Viability Assay Reagent can be stored at -80°C away from light and stable for at least 1 year, or at -20°C away from light and stable for 6 months.

FAD Colorimetric/Fluorometric Assay Kit

Referentie K2042-100 assays

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Description

Quality Control

Components and Storage

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