FM4-64 [162112-35-8]
Referentie HY-103466-5mg
Formaat : 5mg
Merk : MedChemExpress
FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.
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FM4-64 Chemical Structure
CAS No. : 162112-35-8
This product is a controlled substance and not for sale in your territory.
Based on 10 publication(s) in Google Scholar
Description |
FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers. |
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In Vitro |
1. Preparation of FM working solution MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
607.51 |
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Formule |
C30H45Br2N3 |
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CAS No. |
162112-35-8 |
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Appearance |
Solid |
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Color |
Gray to dark gray |
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Emission (Em) |
634 |
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Excitation (Ex) |
558 |
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SMILES |
CC[N+](CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(N(CC)CC)C=C2)C=C1)(CC)CC.[Br-].[Br-] |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : 50 mg/mL (82.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.94% |
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Références |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.6461 mL | 8.2303 mL | 16.4606 mL | 41.1516 mL |
5 mM | 0.3292 mL | 1.6461 mL | 3.2921 mL | 8.2303 mL | |
10 mM | 0.1646 mL | 0.8230 mL | 1.6461 mL | 4.1152 mL | |
15 mM | 0.1097 mL | 0.5487 mL | 1.0974 mL | 2.7434 mL | |
20 mM | 0.0823 mL | 0.4115 mL | 0.8230 mL | 2.0576 mL | |
25 mM | 0.0658 mL | 0.3292 mL | 0.6584 mL | 1.6461 mL | |
30 mM | 0.0549 mL | 0.2743 mL | 0.5487 mL | 1.3717 mL | |
40 mM | 0.0412 mL | 0.2058 mL | 0.4115 mL | 1.0288 mL | |
50 mM | 0.0329 mL | 0.1646 mL | 0.3292 mL | 0.8230 mL | |
60 mM | 0.0274 mL | 0.1372 mL | 0.2743 mL | 0.6859 mL | |
80 mM | 0.0206 mL | 0.1029 mL | 0.2058 mL | 0.5144 mL |
FM4-64 Related Classifications
- Others
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
FM4-64162112-35-8Fluorescent Dyemembraneselectiveredfluorescentdyeendocytosisexocytosisorganelleorganization ,vesicleInhibitorinhibitorinhibit