Galactose Colorimetric Detection Kit
Referentie OKAU00039
Formaat : 2plate
Merk : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Galactose Colorimetric Detection Kit (OKAU00039) |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||||||||||
| ELISA Kit Linearity | Linearity was determined in serum samples by taking two diluted samples with known galactose concentrations, one sample with a high galactose concentration of 5.05 mg/dL and one with a lower value of 2.83 mg/dL, mixing in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.
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| ELISA Kit Principle | The DetectX® Galactose Colorimetric Detection Kit is designed to quantitatively measure galactose in a variety of samples. Please read the complete kit insert before performing this assay. A D-(+)-galactose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of galactose oxidase. The plate is incubated at room temperature for 30 minutes. The galactose oxidase reacts with galactose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorless Substrate to produce a pink-colored product to be read at 560 nm. Increasing levels of galactose cause a linear increase in color. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Three diluted spiked samples were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:
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| ELISA Kit Component |
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| Additional Information | Background: Galactose is a hexose sugar that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Present as an anomeric mixture of a-D-galactose and b-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables. Absorption of galactose in humans is mediated by the Na+/glucose co-transporters SGLT1 and SGLT2 from food across the brush border membrane of the proximal jejunum and renal epithelium. Other sources of galactose include endogenous production and natural turnover of glycolipids and glycoproteins. Adult humans can produce up to 2 grams of galactose per day. Galactose Inside the cells, b-D-galactose is epimerized to a-D-galactose through the action of a mutarotase. a-Dgalactose is subsequently converted to galactose-1-phosphate (Gal-1-P) by the enzyme galactokinase. In the presence of galactose-1-phosphate uridylyltransferase, Gal-1-P reacts with UDP-glucose to form UDPgalactose and glucose-1-phosphate. Glucose-1-phosphate produced can enter the glycolytic pathway or react with UTP in the presence of UDP-glucose pyrophosphorylase to form a new molecule of UDP-glucose. This enzyme pathway comprises the evolutionarily conserved Leloir pathway of galactose metabolism. If the flow of galactose through the Leloir pathway is perturbed either due to congenital deficiency of any of the abovementioned enzymes or an overwhelming presence of this hexose, toxicity syndromes (galactosemia) will be observed. Its cause as a defect in galactose metabolism was identified by a group led by Kalckar in 1956. | ||||||||||||||||||||||||||||||
| :: | Detection Limit: 0.383 mg/dL | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Buffers and TCM | ||||||||||||||||||||||||||||||
| Sensitivity | 0.493 mg/dL |
| Gene Full Name | Galactose Colorimetric |
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