GFAP Chicken Polyclonal Antibody

CAT#: AP31806PU-N

GFAP chicken polyclonal antibody, Aff - Purified


Product Images

Specifications

Product Data
Applications IF, IHC, WB
Recommended Dilution Western Blot.
Immunocytochemistry.
Immunohistochemistry.
Recommended Dilutions:
1/10000-1/20000 for Western blots.
1/2000-1/5000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
Quality Control: This antibody was analyzed by Immunohistochemistry (at dilution of 1/5000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivities Human, Mouse, Rat
Host Chicken
Isotype IgY
Clonality Polyclonal
Immunogen Recombinant Human GFAP (expressed in bacteria).
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
Specificity Recognizes Glial Fibrillary Acidic Protein (GFAP).
Formulation 10 mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified (filter sterilized) IgY fraction.
Concentration 2 mg/ml (based on absorbance at 280 nm)
Purification Affinity Chromatography using a peptide column.
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Gene Name glial fibrillary acidic protein
Background Human GFAP is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
Synonyms Glial Fibrillary Acidic Protein
Reference Data
Protein Families ES Cell Differentiation/IPS

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