GFAP (Glial Fibrillary Acidic Protein) (AP)

Glial fibrillary acidic protein is specific to astrocytes and ependylmal cells of the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Many types of neural tumors such as neuroblastomas Schwannamoas, as well as extra CNS tumors are not labeled.||Applications:|Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilution:|Immunoprecipitation (Native and denatured): 2ug/mg protein lysate (Use Protein G).|Western Blot: 1-2ug/ml for 2 hrs at RT|Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. No special pretreatment is required for staining of formalin fixed tissue.|Optimal dilutions to be determined by the researcher.||Source: Ascites||Positive Control: Astrocytoma, IMR5 and brain cells.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ||Note: Applications are based on unconjugated antibody.