Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Referentie E-BC-K096-M-96T

Formaat : 96T

Merk : Elabscience

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Detection Principle Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Detection Principle Image
Synonyms GSH-PX
Sample Type Serum,Plasma,Cell,Cell culture supernatant,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (400-420 nm, optimum wavelength: 412 nm)
Research Area Oxidative Stress ,  Plant Physiology Series ,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 34.34 U
Detection Range 34.34-1036.64 U
Precision inter-assay CV: 8.7% | intra-assay CV: 2.4%
Assay Time 40 min
Cat.No. Product Name Sizes
E-BC-K030-M Reduced Glutathione (GSH) Colorimetric Assay Kit 96T , 48T
E-BC-K097-M Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit 96T
E-BC-K099-S Glutathione Reductase (GR) Activity Assay Kit 100Assays , 50Assays
E-BC-K278-S Glutathione-S-Transferase (GST) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Mouse serum 3-5
Rat serum 5-8
10% Mouse brain tissue homogenate 1
10% Rat liver tissue homogenate 30-60
HepG2 cells (5 mgprot/mL) 1
10% Epipremnum aureum leaves tissue homogenate 1
10% Chinese cabbage leaves tissue homogenate 3-5

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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