Heat Shock Cognate Protein 70 (HSC70, HSP73)

Referentie H1831-25M-100ul

Formaat : 100ul

Merk : US Biological

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H1831-25M Heat Shock Cognate Protein 70 (HSC70, HSP73)

Clone Type
Polyclonal
Host
mouse
Source
mouse
Swiss Prot
P63017
Isotype
IgM
Grade
Ascites
Applications
FC IC IF IHC IP N WB
Crossreactivity
Bo Fe Hu Mk Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

HSC70-interacting protein (Hip) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of the heat shock protein HSC70 in eukaryotic cells. Hip interacts with the ATPase domain of HSC70 molecules through the tetratricopeptide repeats and flanking charged a-helices of Hip. This binding reaction is dependent on HSP40 in the presence of ATP and becomes Hsp40-independent if HSC70 is preincubated with ADP. Upon binding to HsSC70, Hip stabilizes the ADP-bound state of HsSC70, a conformation that has a high affinity for unfolded substrate proteins. Hip by itself binds unfolded polypeptides, suggesting that it may possess chaperone activity. Together with HSP40, Hip may regulate the chaperone activity of HSC70 by stabilizing the chaperone- substrate complex. ||Applications:|Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Blocking/Neutralization and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:1000 -1:5000|Immunohistochemistry (frozen and paraffin): 1:20-1:200|Flow Cytometry: 1:10 - 1:1000|Immunoprecipitation: 2ul|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgM|Clone No: 6D447 (13D3)|Host: mouse|Source: mouse|Concentration: Not Determined |Form: Supplied as a liquid in PBS, 0.05% sodium azide.|Purity: Ascites|Immunogen: Mouse spermatogenic cell protein.|Specificity: Recognizes mouse HSC70 (heat shock cognate protein 70) at ~70kD by Western Blot. Species Crossreactivity: human, monkey, bovine and feline. Slight cross-reactivity to HSP 70.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse spermatogenic cell protein.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Ascites
Specificity
Recognizes mouse HSC70 (heat shock cognate protein 70) at ~70kD by Western Blot. Species Crossreactivity: human, monkey, bovine and feline. Slight cross-reactivity to HSP 70.
References
Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells: M. Maekawa, et al.; Biol. Reprod. 40, 843 (1989)