Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.
 
> Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody

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Referentie GTX122653-100ul

Formaat : 100ul

Merk : GeneTex

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Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in IHC (Paraffin sections) (IHC-P). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Dot blot (Dot). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot

Reactivity

 Human, Mouse, Rat
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX122653 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Positive Control

NIH-3T3 , BCL-1 , PC-12 , HeLa

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5/Lys8/Lys12/Lys16 of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in IHC (Paraffin sections) (IHC-P). GTX122653

GTX122653 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)(GTX122653) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Dot blot (Dot). GTX122653

GTX122653 Dot Image

Dotblot analysis of anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) at different dilution as indicated.
A: Peptide samples of Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)
B: Peptide samples of Histone H4

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX122653 diluted at 1:10000

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
GTX122653 diluted at 1:10000

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122653

GTX122653 ICC/IF Image

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
15% SDS PAGE
GTX122653 diluted at 1:10000

Application Reference

Hasebe T et al. Gen Comp Endocrinol 2020; 292 : 113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Application : IHC-P
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2) : 118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.
Application : WB
Reactivity : Human
Bai B et al. Birth Defects Res 2018; (Epub) CBP/p300 inhibitor C646 prevents high glucose exposure induced neuroepithelial cell proliferation.
Application : WB
Reactivity : Mouse
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human

REVIEW

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody Cat. No. GTX122653

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : TGW cells
Amount used : 30μg
Blocking : 5% Skim milk in TBST(0.1% tween-20), 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Application Reference
Hasebe T et al. Gen Comp Endocrinol 2020; 292:113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Application : IHC-P
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2):118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.
Application : WB
Reactivity : Human
Bai B et al. Birth Defects Res 2018; (Epub) CBP/p300 inhibitor C646 prevents high glucose exposure induced neuroepithelial cell proliferation.
Application : WB
Reactivity : Mouse
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

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