Hoechst 33258 analog 3 [23554-98-5]
Referentie T19005-100mg
Formaat : 100mg
Merk : TargetMol
Hoechst 33258 analog 3
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Purity:99.4%
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COA HNMR HPLC
Hoechst 33258 analog 3
Catalog No. T19005Cas No. 23554-98-5
Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA.
With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs. Pack Size | |
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
Description | Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA. |
In vitro | The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around an emission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage, the solutions are instead frozen at ≤-20 °C. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. |
Molecular Weight | 422.52 |
Formula | C26H26N6 |
Cas No. | 23554-98-5 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||
Solubility Information | DMSO: 3.57 mg/mL (8.45 mM), Sonication and heating to 70℃ are recommended. | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSO
main solution, add 300 μLPEG300
mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O
mix well and clarify
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
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