Application
| WB, IHC, ICC, IP, E, GSA |
---|---|
Primary Accession | P38532 |
Other Accession | NP_032322.1 |
Host | Rat |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine, Guinea Pig |
Clonality | Monoclonal |
Description | Rat Anti-Mouse HSF1 Monoclonal IgG1 |
Target/Specificity | Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates). |
Other Names | HSTF1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody |
Clone Names | 4B4 |
Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-477 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody. |
Cellular Localization | Cytoplasm | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
References
1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.
2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.
3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200.
4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.
5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528.
6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.
7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474.
8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.
9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.
10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343.
11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.