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Western Blot (Tissue lysate)
Note

Application Data with Unconjugated Antibody

Western Blot (Tissue lysate)

Western blot analysis of rat brain membrane tissues with Hsp90b1 polyclonal antibody (PAB28946) at 1:250 dilution.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of Hsp90b1.

    Immunogen

    A synthetic peptide corresponding to amino acids at C-terminus of mouse Hsp90b1.

    Host

    Rabbit

    Reactivity

    Bovine, Human, Mouse, Rat

    Specificity

    Detects ~94 kDa.

    Form

    Liquid

    Conjugation

    HRP

    Purification

    Peptide Affinity Purified

    Recommend Usage

    Immunocytochemistry
    Immunohistochemistry
    Immunoprecipitation (1:80)
    Western blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)

    Storage Instruction

    Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody

    Western Blot (Tissue lysate)

    Western blot analysis of rat brain membrane tissues with Hsp90b1 polyclonal antibody (PAB28946) at 1:250 dilution.

  • Applications

    Western Blot

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

  • Gene Info — Hsp90b1

    Entrez GeneID

    22027

    Protein Accession#

    P08113

    Gene Name

    Hsp90b1

    Gene Alias

    ERp99, GRP94, TA-3, Targ2, Tra-1, Tra1, gp96

    Gene Description

    heat shock protein 90, beta (Grp94), member 1

    Gene Ontology

    Hyperlink

    Gene Summary

    beta (Grp94)

    Other Designations

    90 kDa|OTTMUSP00000022393|endoplasmin|heat shock protein 90kDa beta (Grp94), member 1|transforming growth factor alpha regulated gene 2|tumor rejection antigen (gp96) 1|tumor rejection antigen gp96