Human Cathepsin D antibody

Product Information

Product Type

Monoclonal Antibody

Clone Number

4G2

UniProt No.

P07339

NCBI Accession No.

NP_001900

Alternative names

CTSD, CPSD, Cathepsin D, Lysosomal aspartyl protease, CLN10, Ceroid-lipofuscinosis, Neuronal 10

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Cathepsin D (21-412 aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, IHC

Usage

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate

The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ

Immunohistochemistry (IHC)

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)