Date : Anonymous submitted on 03-Jan-2021

Score :

Application Tested : IHC-P

Figure Note : Healthy adult human retina sections (GTX22016) were deparaffinized and rehydrated. For antigen retrieval, sections were incubated in a heated (95ºC) Dako Target Retrieval Solution, pH 9 for 20 min. Samples were permeabilized by 0.5% Triton X for 15 min and blocked with 5% BSA for 1 hour at RT. Retinal microglia was detected by incubation with IBA-1 (1:400) and visualized by Donkey anti-rabbit Cy3 (1:1000). Nuclei counterstained by DAPI.
The white arrow indicates nuclei in the Ganglion cell layer.
The yellow arrow indicates the retinal vessel.
GCL – ganglion cells layer; IPL – inner plexiform layer; INL - inner nuclear layer; ONL - Outer plexiform layer, PR – photoreceptors.