Human HDAC4 (Histone Deacetylase 4) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	HDAC4
Gene ID:	9759
Uniprot ID:	HDAC4_HUMAN
Sample Type:	tissue homogenates or other biological fluids.

Tissue Specificity	Ubiquitous.
Post Translational Modifications	Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1.
Protein Name	Histone Deacetylase 4 Hd4
Database Links	Reactome: R-HSA-2122947 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-350054 Reactome: R-HSA-4090294 Reactome: R-HSA-4551638 Reactome: R-HSA-8941284 Reactome: R-HSA-8951936
Cellular Localisation	Nucleus Cytoplasm Shuttles Between The Nucleus And The Cytoplasm Upon Muscle Cells Differentiation It Accumulates In The Nuclei Of Myotubes Suggesting A Positive Role Of Nuclear Hdac4 In Muscle Differentiation The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-246 Ser-467 And Ser-632 By Camk4 And Sik1 The Nuclear Localization Probably Depends On Sumoylation Interaction With Sik3 Leads To Hdac4 Retention In The Cytoplasm
Alternative ELISA Names	Histone Deacetylase 4 ELISA kit Hd4 ELISA kit HDAC4 ELISA kit KIAA0288 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance