Human IFNg (Interferon Gamma) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	IFNG
Gene ID:	3458
Uniprot ID:	IFNG_HUMAN
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Tissue Specificity	Released primarily from activated T lymphocytes.
Post Translational Modifications	Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Function	Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Protein Name	Interferon Gamma Ifn-Gamma Immune Interferon
Database Links	Reactome: R-HSA-877300 Reactome: R-HSA-877312 Reactome: R-HSA-8877330 Reactome: R-HSA-8950505 Reactome: R-HSA-9732724
Cellular Localisation	Secreted
Alternative ELISA Names	Interferon Gamma ELISA kit Ifn-Gamma ELISA kit Immune Interferon ELISA kit IFNG ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance