Human IgG4 (Fc) monoclonal antibody, clone RM217 (Biotin)
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- More Files
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat # MAB12822) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against of human IgG4 (Fc).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to the Fc region of human IgG4.
Sequence
N/A
Reactivity
Human
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
Flow Cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG4 (Fc) monoclonal antibody, clone RM217 (Cat # MAB12822) at the following concentrations: 0.2 ug/mL, 0.1 ug/mL, 0.05 ug/mL, 0.025 ug/mL, 0.013 ug/mL, 0.006 ug/mL, 0.003 ug/mL. The plate was coated with 50 uL/well different amounts of human IgG4. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
- Gene Info — IGHG4
- Interactomes