Human sICAM-1 ELISA Kit (96-well)
Referentie EA100070
Formaat : 1x96wells
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| Product Data | |
| Species | Human |
|---|---|
| Format | 96-well strip plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hours |
| Signal | Colorimetric |
| Curve Range | 62.5-4000pg/ml |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Specificity | Natural and recombinant Human sICAM-1 Ligand |
| Sensitivity | 31pg/mL |
| Reactivity | Human |
| Interference | No significant interference observed with available related molecules. |
| Components |
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| Background | Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein that plays a key role in leukocyte migration and activation . Human ICAM-1 contains five Ig-like domains in its extracellular domain (ECD) and associates into non-covalently linked dimers . Soluble forms of monomeric and dimeric ICAM-1 (sICAM-1) can be generated via proteolytic cleavage by cathepsin G, elastase, MMP-9, MMP-14/MT1-MMP, and TACE/ADAM17 . In the mouse, alternate splicing generates isoforms that lack particular Ig-like domains and are differentially sensitive to proteolysis . Within the ECD, human ICAM-1 shares 53% amino acid sequence identity with mouse and rat ICAM-1. The principal binding partners of ICAM-1 are the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . The multivalency of dimeric ICAM-1 increases its strength of interaction with LFA-1 . ICAM-1 also binds several non-integrin ligands including CD43/sialophorin, fibrinogen, hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes . At sites of inflammation, ICAM-1 is upregulated on endothelial and epithelial cells where it mediates the adhesion and paracellular migration of leukocytes expressing activated LFA-1 and Mac-1 . ICAM-1 ligation prolongs antigen presentation by dendritic cells and promotes T cell proliferation and cytokine release . ICAM-1 activation also participates in angiogenesis, wound healing, and bone metabolism . Soluble ICAM-1 has been reported in serum, cerebrospinal fluid, urine, and bronchoalveolar lavage fluid . Elevated levels of sICAM-1 in these fluids are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies . sICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage . It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis . |
| Gene Symbol | ICAM1 |
| Locus ID | 3383 |
| Standard Curve |  |
| Shipping | Blue Ice |
| SDS | |