Human TNF alpha ELISA
Referentie DIY2096H-003
Formaat : 1Set
Merk : Kingfisher Biotech
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Telefoonnummer : +1 850 650 7790
Human TNFα ELISA Data
Human TNFα ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Canine TNFα (TNFSF2) Polyclonal Antibody | Capture Antibody | 100 µg | PB0389D-100 |
| Biotinylated Anti-Canine TNFα (TNFSF2) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0397D-050 |
| Human TNFα (TNFSF2) Recombinant Protein | Standard | 5 µg | RP0440H-005 |
Specifications
The Human TNFα (TNFSF2) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Human TNFα ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Human TNFα Do-It-Yourself ELISA can also be used to measure Chimpanzee TNFα.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Background
TNFα (TNFSF2) is a member of the TNF Superfamily. It is produced chiefly by activated macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue. The primary role of TNFα is in the regulation of immune cells. TNFα, being an endogenous pyrogen, is able to induce fever, to induce apoptotic cell death, to induce sepsis (through IL-1 & IL-6 production), to induce cachexia, induce inflammation, and to inhibit tumorigenesis and viral replication.
Alternate Names - TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-α, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha