PC2007-25ml | HyperChrom AR rProtein A/G Agarose 4FF Agarose Clinisciences

Description

HyperChrom AR rProtein A/G Agarose 4FF Agarose is an affinity chromatography medium for antibody purification, separated by specific interaction between antigens and antibodies, mainly used for the purification of IgG in culture media, serum, ascites or hybridoma cell culture supernatant, or the purification of recombinant proteins containing Fc fragments.

The HyperChrom AR rProtein A FF Agarose and HyperChrom Protein G 4FF Agarose in this product are covalently attached to 4% high crosslinked, high flow rate agarose and mixed in a 1:1 ratio. Each milliliter of HyperChrom AR rProtein A/G Agarose 4FF Agoros can bind approximately 20 mg of human IgG.

HyperChrom AR rProtein A/G Agarose 4FF Agarose chromatography media parameters

Name	Description
Chromatography media type	Affinity chromatography media
Ligation	rProtein A/G (E. coli recombinant expression, 1:1).
Scaffolding	Highly cross-linked agarose
Average particle size	90 μm
Dynamic load	~20 mg human IgG/mL chromatography medium
Flow rates are recommended	50-400 cm/h
Withstand pressure	0.3 MPa
Use temperature	4-30℃
pH stability	3-9

Quality Control

Protocol

Components and Storage

Components	PC2007-25 mL	PC2007-100 mL
HyperChrom AR rProtein A/G Agarose 4FF Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom AR rProtein A/G Agarose 4FF Agarose

Referentie PC2007-25ml

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Description

Quality Control

Components and Storage