PC2013-10ml | HyperChrom DEAE HP Agarose Clinisciences

Description

HyperChrom DEAE HP Agarose is a weak anion exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom DEAE HP Agarose chromatography media exhibits unexpected separation and purification effects. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.

HyperChrom DEAE HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (3) Fine particle size design to improve resolution.

HyperChrom DEAE HP Agarose chromatography media parameters

Name	Description
Chromatography media type	Weak anion exchange
Ligation	-O-CH₂CH₂–N⁺ (C₂H₅)₃H
Scaffolding	Highly cross-linked agarose
Average particle size	34 μm
Ion load	0.08-0.13 mmol Cl^-/mL chromatography medium
Dynamic load	>90 mg ovalbumin/mL chromatography medium^*
Flow rates are recommended	90-150 cm/h
Maximum flow rate	200 cm/h
Withstand pressure	0.3 MPa
Use temperature	4-30℃
pH stability ^**	2-14
Solvent resistant	Common aqueous solution, 30% isopropanol^*, 75% ethanol^*, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents	Oxidant, anionic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 150 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml ovalbumin sample, ovalbumin loading volume per volume of media (mL) (mg) when ovalbumin penetration reaches 10%. After 7 days in the environment of chromatography at 40 °C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. *30% and 75% are v/v, volume ratio.

Quality Control

Protocol

Components and Storage

Components	PC2013-25 mL	PC2013-100 mL
HyperChrom DEAE HP Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom DEAE HP Agarose

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Description

Quality Control

Components and Storage