PC2011-10ml | HyperChrom Q HP Agarose Clinisciences

Description

HyperChrom Q HP Agarose is a strong anion exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.

HyperChrom Q HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (3) Fine particle size design, with high resolution.

HyperChrom Q HP Agarose chromatography media parameters

Name	Description
Chromatography media type	Strong anion exchange
Ligation	-N+(CH3)₃(quaternary ammonium).
Scaffolding	Highly cross-linked agarose
Average particle size	34 μm
Ion load	0.14-0.20 mmol Cl-/mL chromatography medium
Dynamic load	> 70 mg BSA/mL chromatography medium^*
Flow rates are recommended	90-150 cm/h
Maximum flow rate	200 cm/h
Withstand pressure	0.3 MPa
Use temperature	4-30℃
pH stability ^**	2-14
Solvent resistant	Common aqueous solution, 30% isopropanol^*,75% ethanol^*, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents	Oxidant, anionic detergent
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml BSA sample, BSA loading volume per unit volume (mL) (mg) when the penetration of BSA reaches 10%. **After 7 days in the environment of chromatography at 40 °C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. 30% and 75% are v/v, volume ratio.

Quality Control

Protocol

Components and Storage

Components	PC2011-25 mL	PC2011-100 mL
HyperChrom Q HP Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom Q HP Agarose

Referentie PC2011-10ml

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Description

Quality Control

Components and Storage