ICAM1 ELISA Kit (Dog)
Referentie OKCA02020
Formaat : 96Wells
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Dog | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ICAM1 ELISA Kit (Dog) (OKCA02020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ICAM1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 25 ng/mL-1,600 ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay Variation <10% CV (n=20) Mean Inter-Assay Variation <12% CV (n=8) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 6.25 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | ICAM1 |
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| Alias Symbols | ICAM-1 |
| NCBI Gene Id | 403975 |
| Protein Name | Intercellular adhesion molecule 1 |
| Description of Target | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Uniprot ID | P33729 |
| Protein Accession # | NP_001003291 |
| Nucleotide Accession # | NM_001003291 |