IHC-plus™ TAGLN / Transgelin / SM22 Antibody (aa190-201)
Referentie LS-B2563-50
Formaat : 50ug
Merk : LSBio
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Telefoonnummer : +1 850 650 7790
Overview
Antibody:
TAGLN / Transgelin / SM22 Goat anti-Human Polyclonal (aa190-201) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
SM22 antibody LS-B2563 is an unconjugated goat polyclonal antibody to SM22 (TAGLN / Transgelin) (aa190-201) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
(4) Publication(s)
Target
Human TAGLN / Transgelin / SM22
Synonyms
TAGLN | 22 kDa actin-binding protein | SMCC | TAGLN1 | WS3-10 | Transgelin | Transgelin variant 2 | SM22 | Protein WS3-10 | SM22-alpha
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-MTGYGRPRQIIS, from the C-Terminus of protein sequence according to NP_003177.2.
Epitope
aa190-201
Specificity
Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
- Western blot (0.01 - 0.03 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Immunohistochemistry: LS-B2563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2563 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About TAGLN / Transgelin / SM22
Q01995 NM_003186 NP_003177.2
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@clinisciences.com