Insulin (INS) BioAssay™ ELISA Kit (Canine)

This Insulin (INS) BioAssay™ employs the competitive inhibition enzyme immunoassay technique for detection of canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids||Specificity:|This assay has high sensitivity and excellent specificity for detection of Insulin (INS). No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.||Precision:|Intra-Assay: CV<10% |Inter-Assay: CV<12%||Assay Principle:|A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.||Kit Components:|96-well strip plate, 1x Plate |Standard, 2x vial|Detection Reagent A, 1|Detection Reagent B, 1×120ul|Reagent Diluent, 1×300ul|TMB Substrate, 1×9ml|Wash Buffer (30X), 1×20ml|Standard Diluent, 1×20ml|Assay Diluent A, 1×12ml|Assay Diluent B, 1×12ml|Stop Solution, 1×6ml||Storage and Stability:|Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.