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IPTG [367-93-1]

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Referentie HY-15921-500mg

Formaat : 500mg

Merk : MedChemExpress

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IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

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IPTG Chemical Structure

IPTG Chemical Structure

CAS No. : 367-93-1

This product is a controlled substance and not for sale in your territory.

Based on 11 publication(s) in Google Scholar

    IPTG purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 May 7;19(5). pii: E1393.  [Abstract]

    Screening of thymidylate synthase (TYMS) overexpression conditions.
    Description

    IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

    In Vitro

    IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    IPTG Related Antibodies
    Masse moléculaire

    238.30

    Formule

    C9H18O5S
    CAS No.

    367-93-1
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1SC(C)C
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvant et solubilité
    In Vitro: 

    H2O : 100 mg/mL (419.64 mM; Need ultrasonic)

    DMSO : ≥ 60 mg/mL (251.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1964 mL 20.9820 mL 41.9639 mL
    5 mM 0.8393 mL 4.1964 mL 8.3928 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureté et documentation

    Purity: 99.90%

    SDS (393 KB)

    COA (252 KB) HNMR (246 KB) RP-HPLC (194 KB) MS (193 KB)

    Instruction de manipulation (2659 KB)
    Références

    • [1]. Isopropyl β-D-1-thiogalactopyranoside

    • [1]. Isopropyl β-D-1-thiogalactopyranoside

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.1964 mL 20.9820 mL 41.9639 mL 104.9098 mL
    5 mM 0.8393 mL 4.1964 mL 8.3928 mL 20.9820 mL
    10 mM 0.4196 mL 2.0982 mL 4.1964 mL 10.4910 mL
    15 mM 0.2798 mL 1.3988 mL 2.7976 mL 6.9940 mL
    20 mM 0.2098 mL 1.0491 mL 2.0982 mL 5.2455 mL
    25 mM 0.1679 mL 0.8393 mL 1.6786 mL 4.1964 mL
    30 mM 0.1399 mL 0.6994 mL 1.3988 mL 3.4970 mL
    40 mM 0.1049 mL 0.5245 mL 1.0491 mL 2.6227 mL
    50 mM 0.0839 mL 0.4196 mL 0.8393 mL 2.0982 mL
    60 mM 0.0699 mL 0.3497 mL 0.6994 mL 1.7485 mL
    80 mM 0.0525 mL 0.2623 mL 0.5245 mL 1.3114 mL
    100 mM 0.0420 mL 0.2098 mL 0.4196 mL 1.0491 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    IPTG Related Classifications

    • Others
    • Others
    • Biochemical Assay Reagents
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    Keywords:

    IPTG367-93-1Isopropyl β-D-thiogalactosideBiochemical Assay ReagentsInhibitorinhibitorinhibit