Isocitrate BioAssay™ Kit (Isocitrate Acid)

Isocitrate (Isocitrate Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly|found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. The isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly|fluorescent product. The fluorescence intensity of this product, measured at lex/em= 530/585nm, is proportional to the isocitrate concentration in the sample.||Intended Use:|For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.||Applications:|Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) ||Range:|0.6-500um for 10 min reaction.||Detection limit: |0.6um||Kit Components:|Assay Buffer, 1x10ml|NADP, 1x500ul|Standard, 1x1ml|Enzyme A, 1x120ul|Enzyme B, 1x120ul|Probe, 1x750ul||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.