K2167-100 | Lipid Peroxidation (MDA) Assay Kit Clinisciences

Description

Malondialdehyde (MDA) is one of the products of lipid peroxidation in organisms, and it is widely used as a detection indicator for lipid peroxidation.

The Lipid Peroxidation (MDA) Assay Kit is a kit for the detection of MDA in samples such as tissues, cell lysates, plasma, serum, and urine by thiobarbituric acid (TBA). The detection principle is that MDA reacts with TBA to produce a red product that is specifically absorbed at 535 nm and can therefore be determined by colorimetry. At the same time, the reaction products can also be excited at 535 nm, resulting in a maximum emission wavelength of 553 nm, so fluorescence detection can also be performed.

The kit provides antioxidants that can inhibit the production of new MDA in the sample during the detection process, making the detection more accurate. At the same time, the kit can detect MDA as low as 1 μM and has a good linear relationship in 1-200 μM.

Figure 1: Reference standard curve of this kit

Quality Control

Protocol

Components and Storage

Components	K2167-100 T	K2167-500 T
TBA	25 mg	125 mg
TBA Preparation Buffer	7 mL	35 mL
TBA Dilution Buffer	15 mL	75 mL
Antioxidant	300 μL	1.5 mL
MDA Standard (1 mM)	200 μL	1 mL
Store the kit at -20°C, stable for 1 year. TBA and the antioxidant should be stored away from light.

Lipid Peroxidation (MDA) Assay Kit

Referentie K2167-100

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Description

Quality Control

Components and Storage

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