LSP1 (phospho S252) polyclonal antibody
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- More Files
Western Blot
The whole cell lysate derived from m-CSF stimulated Jurket cells (Lane 1) or mouse spleen (Lane 2) were immune-probed by LSP1 (phospho S252) polyclonal antibody (Cat # PAB12633) at 1 : 500. An immunoreactive band is observed around ~ 52 KDa.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of LSP1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S252 of human LSP1.
Host
Rabbit
Theoretical MW (kDa)
52
Reactivity
Human, Mouse
Specificity
This antibody recognizes ~52 KDa of human LSP1 at a phosphorylated serine 252. It does not recognize non-phosphorylated peptides confirmed by dot blot.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot
The whole cell lysate derived from m-CSF stimulated Jurket cells (Lane 1) or mouse spleen (Lane 2) were immune-probed by LSP1 (phospho S252) polyclonal antibody (Cat # PAB12633) at 1 : 500. An immunoreactive band is observed around ~ 52 KDa.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
- Gene Info — LSP1
Entrez GeneID
4046Gene Name
LSP1
Gene Alias
WP34, pp52
Gene Description
lymphocyte-specific protein 1
Omim ID
153432Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
47 kDa actin binding protein|52 kDa phosphoprotein|F-actin binding and cytoskeleton associated protein|OTTHUMP00000014139|OTTHUMP00000069612|leufactin (leukocyte F-actin binding protein)|leukocyte-specific protein 1|lymphocyte-specific antigen WP34|lympho
- Interactomes
- Diseases
- Publication Reference
- LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration.
Liu L, Cara DC, Kaur J, Raharjo E, Mullaly SC, Jongstra-Bilen J, Jongstra J, Kubes P.
The Journal of Experimental Medicine 2005 Feb; 201(3):409.
- LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration.