Macrophages (PE)

Referentie M1250-01C-PE-100ul

Formaat : 100ul

Merk : US Biological

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M1250-01C-PE Macrophages (PE)

Clone Type
Polyclonal
Host
mouse
Source
porcine
Isotype
IgG2b
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.||Applications:|Suitable for use in Flow Cytometry (Not Tested), Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. ||Recommended Dilutions:|Flow Cytometry (Not Tested): 1:50-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.|Immunohistochemistry: Frozen sections|Western Blot: Non-reducing conditions.|Optimal dilutions to be determined by the researcher. ||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice.||Recommended Negative Control:|I1904-84Q3: IgG2b Murine Negative Control||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: BA4D5|Host: mouse|Source: porcine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Porcine alveolar macrophages|Specificity: Recognizes porcine cells of the monocyte/macrophage lineage. Stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer’s patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. Recognizes a 105kD antigen in pig macrophages.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Porcine alveolar macrophages
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine cells of the monocyte/macrophage lineage. Stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer’s patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. Recognizes a 105kD antigen in pig macrophages.
References
1. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation. Eur J Cell Biol. 87: 91-9. 2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update. Vet Res. 39: 54.