Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Referentie E-BC-K025-S-100

Formaat : 100Assays

Merk : Elabscience

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Detection Principle MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Synonyms MDA
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (532 nm)
Research Area Oxidative Stress
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.38 nmol/mL
Detection Range 0.38-133.33 nmol/mL
Precision inter-assay CV: 0.08 | intra-assay CV: 4.9%
Assay Time 1 h 30 min
Cat.No. Product Name Sizes
E-BC-K031-S Catalase (CAT) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human plasma 1
Rat serum 1
Rat plasma 1
Mouse serum 1
Mouse plasma 1
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat brain tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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