MG-63
Referentie 300441
Formaat : 1cryovial
Merk : CLS Cell Lines Service
MG-63 Cells
Basic details about MG-63 cells
Description | MG-63 cells, a human osteosarcoma cell line derived from the bone of a 14-year-old White male patient with osteosarcoma, are a pivotal model in bone biology research. MG63 human osteosarcoma cells, with their fibroblast morphology and rapid proliferation, serve as an essential tool in understanding bone metabolism, particularly in the context of osteosarcoma. MG-63 cells produce high levels of human interferon when induced with agents like polyinosinic acid-polycytidylic acid, cycloheximide, and actinomycin D. Enhanced interferon production is crucial for studies focusing on the immune responses within the bone microenvironment. Seeding MG-63 cells on biocompatible surfaces like Bioglass disks, titanium (Ti-6Al-4V) disks, and cobalt chrome (Co-Cr-Mo) alloys is possible due to their strong cell adherence and attachment. They are a good osteoblastic model for studying osseointegration and bone cell-implant interactions with amorphous carbon films and composite tantalum. Research involving the osteoblastic cell line MG-63 often focuses on apoptosis, the regulation, and expression of osteocalcin, and the impact of adenosine on bone metabolism. Overall, MG-63 cells remain a cornerstone in the study of human osteoblast-like cells, offering insights into cell growth, differentiation, and the intricate interactions between bone cells and their microenvironment. |
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Organism | Human |
Tissue | Bone |
Disease | Osteosarcoma |
Metastatic site | Bone, left femur |
Synonyms | M-G63, MG63 |
Aspects of the human osteosarcoma cell line MG-63
Age | 14 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Specifications
Citation | MG-63 (Cytion catalog number 300441) |
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Biosafety level | 1 |
Genomics of MG63 cells
Receptors expressed | Transforming growth factor beta (TGF beta, type I and type II) |
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Products | Interferon |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Genetic profile
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 TH01: 9.3 TPOX: 8,11 vWA: 16,19 D3S1358: 15 D21S11: 30 D18S51: 16 Penta E: 11,12 Penta D: 9,13 D8S1179: 13 FGA: 21,25 |
HLA alleles | A*: 01:01:01 B*: 08:01:01 C*: 07:01:01 DRB1*: 03:01:01 DQA1*: 05:01:01 DQB1*: 02:01:01 DPB1*: 01:01:01, 04:02:01 E: 01:01:01 |