Application
| IF, IHC-P, WB, E |
|---|---|
| Primary Accession | P09581 |
| Other Accession | Q00495, NP_001032948.2 |
| Reactivity | Mouse, Rat |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 109179 Da |
| Antigen Region | 895-923 aa |
| Gene ID | 12978 |
|---|---|
| Other Names | Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, Csf1r, Csfmr, Fms |
| Target/Specificity | This Mouse Csf1r antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 895-923 amino acids from the C-terminal region of mouse Csf1r. |
| Dilution | IF~~1:25 WB~~1:1000-2000 IHC-P~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Csf1r Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Csf1r |
|---|---|
| Synonyms | Csfmr, Fms |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation |
| Tissue Location | Widely expressed.. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Csf1r is a protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
References
Gueller, S., et al. J. Leukoc. Biol. 88(4):699-706(2010)
Nagamachi, A., et al. Dev. Biol. 345(2):226-236(2010)
Wei, S., et al. J. Leukoc. Biol. 88(3):495-505(2010)
Maitra, R., et al. J. Immunol. 185(3):1485-1491(2010)
Aikawa, Y., et al. Nat. Med. 16(5):580-585(2010)
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Cat# AP13911b