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> Mouse IgG (H&L) antibody (FITC)

Mouse IgG (H&L) antibody (FITC)

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Referentie orb347618-2mg

Formaat : 2mg

Merk : Biorbyt

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    Mouse IgG (H&L) antibody (FITC)

    Catalog Number: orb347618

    Catalog Numberorb347618
    CategoryAntibodies
    DescriptionMouse IgG (H&L) antibody (FITC)
    Species/HostSheep
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Sheep Serum, Mouse IgG and Mouse Serum.
    ConjugationFITC
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namessheep anti-Mouse IgG fluorescein Conjugated Antibo
    NoteFor research use only
    Application notesAnti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Expiration Date12 months from date of receipt.
    Mouse IgG (H&L) antibody (FITC)

    Western Blot of Fluorescein Sheep Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein sheep secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

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