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MSTO-211H growing culture

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Referentie 330450

Formaat : 1cellcultureflask

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : CLS Cell Lines Service

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

MSTO-211H Cells

Description	The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung. The patient had not received prior radiation or chemotherapy.
Organism	Human
Tissue	Lung
Disease	Pleural mesothelioma
Synonyms	MSTO-211 H, MSTO211H, MSTO-211, 211H, MeSoTheliOma-211H

Age	62 years
Gender	Male
Ethnicity	Caucasian
Growth properties	Adherent

Citation	MSTO-211H (Cytion catalog number 300450)
Biosafety level	1

Protein expression	High affinity binding sites for EGF, expression of Neuron specific enolase (NSE) and alpha and beta subunits of HCG, L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
Tumorigenic	Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
Karyotype	modal number = 72, range = 70 to 78

Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Doubling time	20 hours
Subculturing	The cells can reach a saturation density of 400.000 cells per cm2, but will slough off the surface as they attain this density. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	A ratio of 1:3 to 1:6 is recommended
Seeding density	1 x 10^4 cells/cm^2
Fluid renewal	2 to 3 times per week
Freezing recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,y CSF1PO: 11,12 D13S317: 11,14 D16S539: 13 D5S818: 12 D7S820: 8,12 TH01: 8,9.3 TPOX: 11 vWA: 16,18 D3S1358: 15 D21S11: 28,31 D18S51: 16,18 Penta E: 7,13 Penta D: 11,12 D8S1179: 13 FGA: 21
HLA alleles	A: 01:01:01, 03:01:01 B: 07:02:01, 39:01:01 C: 07:02:01, 12:03:01 DRB1: 01:01:01, 04:01:01 DQA1: 01:01:01, 03:01:01 DQB1: 03:02:01, 05:01:01 DPB1*: 04:01:01 E: 01:01, 01:03