Mucin 5AC (MUC5AC)(45M1), 0.2mg/mL
Referentie BNUB0031-500
Formaat : 500uL
Merk : Biotium
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
| Antibody number | #0031 |
|---|---|
| Antibody reactivity (target) | Mucin 5AC |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | 45M1 |
| Isotype | IgG1, kappa |
| Molecular weight | >1,000 kDa |
| Synonyms | Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM) |
| Human gene symbol | MUC5AC |
| Entrez gene ID | 4586 |
| SwissProt | P98088 |
| Unigene | 534332 |
| Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient |
| Antibody target cellular localization | Secreted (extracellular) |
| Verified antibody applications | IHC (FFPE) (verified) |
| Species reactivity | Cat, Chicken, Hedgehog, Human, Monkey, Mouse, Pig, Rabbit, Rat |
| Expected antibody applications | ELISA (published for clone), Flow, surface (published for clone), WB (published for clone) |
| Antibody application notes | Flow cytometry: 0.5-1 ug/million cells in 0.1 mL, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL, Does not react with cow, others not known, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user |
| Positive control | Stomach |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Cancer, Mucins |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Epithelial cells |
| Tumor expression | Ovarian cancer |
Product Description
This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
info@clinisciences.com
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 0031, Anti-Mucin 5AC (45M1)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
References
Note: References for this clone sold by other suppliers may be listed for expected applications.
- Biochem J (2002) 364, 191-200. (IHC, FFPE; ELISA)
- FEBS Journal 275 (2008): 481-489. (WB; epitope mapping)
- Cancer Immunol Immunother (2013) 62: 1011-1019. (Flow, surface)