WESTAR NOVA 2.0 is an ECL substrate with stable light output for low picogram detection level.
The formulation provides a low background for a high signal to noise ratio.
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NeoPRO Pico Western ECL Substrate
Referentie NB-78-00002
Formaat : 2x125mLkit
Merk : Neo Biotech
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Telefoonnummer : +1 850 650 7790
WESTAR NOVA 2.0
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Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR NOVA 2.0 and its competitors
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
WESTAR NOVA 2.0 Signal Duration Comparison with Competitors
Comparison of signal intensities at time points up to 20 hours post substr.ate addition.
Exposure time is 180 seconds for each time point (0-2-5-8 hours).
Exposure time is 180 seconds for each time point (0-2-5-8 hours).
