WESTAR-ONE BASIC is our entry-level pre-mixed chemiluminescent HRP substrate for mid-picogram detection level in Western Blot.
Ideal for routinely analysis. The formulation provides a low background for a high signal to noise ratio.
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NeoPRO RTU Nano - Ready to Use Western ECL Substrate
Referentie NB-78-00007
Formaat : 250mLkit
Merk : Neo Biotech
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
WESTAR-ONE BASIC
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Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR-ONE BASIC and its competitors
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1: 20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare.
Exposure time: 120 seconds
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1: 20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare.
Exposure time: 120 seconds
