Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

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Referentie GTX33916-100ug

Formaat : 100ug

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : GeneTex

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Anti-Angiopoietin 2 antibody [Angy-2-1] used in (). GTX33916

Anti-Angiopoietin 2 antibody [Angy-2-1] used in ELISA (ELISA). GTX33916

Host	Mouse
Clonality	Monoclonal
Clone Name	Angy-2-1
Isotype	IgG2b
Application	ELISA, Neutralizing/Inhibition
Reactivity	Human, Mouse
Package	100 μg

Datasheet File

View product citations for antibody GTX33916 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ELISA	Assay dependent
Neutralizing/Inhibition	Assay dependent
Note : Neutralizing/Inhibition Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) *ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2).

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Product Note

Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human angiopoietin-2.

Purification

Protein A-affinity purified.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

RRID

AB_2887728

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin 2 , AGPT2 , ANG2

Cellular Localization

Secreted

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

GTX33916 Image

GTX33916 ELISA Image

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1μg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 20ng/μl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

Application Reference

Zhong J et al. Phytomedicine 2020; 78 : 153302 Notoginsenoside R1 activates the Ang2/Tie2 pathway to promote angiogenesis.